KMID : 0353320150390020127
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Oral Biology Research 2015 Volume.39 No. 2 p.127 ~ p.134
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Fabrication and cell attachment characteristics of nanotubes on the surface of Ti-6Al-4V dental implant alloy
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Ahn Hoon
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Abstract
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Purpose: The purpose of this study was to investigate cell attachment characteristics of nanotubes on the surface of Ti-6Al-4V dental implant alloy.
Materials and Methods: TiO2 nanotubes were formed on the Ti-6Al-4V alloys by anodic oxidation. The electrolyte solution was prepared by adding H3PO4 solution to 0.8 wt% NaF solution and then stirred with a magnetic stirrer. With a platinum electrode and a saturated calomel electrode, electrochemical experiments were performed using a conventional three electrode configuration. Anodic oxidation was carried out using a scanning potentiostat, and all experiments were proceeded as planed at room temperature. Crystallization treatment of nanotube surface went off at 450oC for 3 hours. MC3T3-E1 cell culture was performed with MC3T3-E1 mouse osteoblast for 2 days.
Results: Microstructure of the alloys and nanotube surface were observed by optical microscope, field emission scanning electron microscope, and crystallization of nanotube was identified by X-ray diffractometer.
Conclusion: The formation of the nanotube structure on the Ti-6Al-4V alloy significantly increased the surface area, which could be useful for the adsorption and adhesion enhancements of cells and could be applied for drug delivery system.
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KEYWORD
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Cell attachment characteristics, MC3T3-E1 mouse osteoblast, Nanotube, Ti-6Al-4V dental implant alloy
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